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KMID : 0903519890320020148
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1989 Volume.32 No. 2 p.148 ~ p.153
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Genetic Breeding of Korean Soybean Paste - Fermenting Bacillus sp . by UV Mutation
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Abstract
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Several mutants for rapid fermentation of Korean soybean paste which will improve the productivity of amylase and protease was obtained through the second mutation of the original strain using UV radiation. The original strain was the VTG treated mutant of the Bacillus sp. producing peculiar flavour which had been isolated from the Korean soybean paste. A mutant (SSA3-2M1) could improve the productivity of amylase by 4.4 times and that of protease by 3.7 times. Other one (SSA3-2M2) depressed deaminase productivity by 90% in spite of improvement of amylase and protease. The enzymes produced by strains were similar in .enzymatic properties such as optimal reaction pH and temperature. The reaction and productivity of enzymes were not influenced in the high concentration of salt.
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